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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Bios Logos Jurnal Natur Indonesia Jurnal Keteknikan Pertanian AL KAUNIYAH Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Penelitian Kehutanan Sumatrana Jurnal Riau Biologia Jurnal Pengabdian UntukMu NegeRI BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Bio-Lectura Jurnal Agronomi Tanaman Tropika (JUATIKA) Dinamika Pertanian
Dewi Indriyani Roslim
Universitas Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences

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Journal : Dinamika Pertanian

 1 
RESPON GENOTIPE UBI KAYU (Manohot Esculenta Crantz) TERHADAP DOSIS PUPUK KANDANG KOTORAN SAPI TALUK KUANTAN Herman Herman; Dewi Indriyani Roslim; Ingga Yurisna Fitriani
DINAMIKA PERTANIAN Vol. 32 No. 2 (2016): Jurnal Dinamika Pertanian Edisi Agustus 2016
Publisher : UIR Press

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Abstract

Cassava (Manihot esculenta crantz) is one of the agricultural commoditythat produce tuber for food source in Indonesia. The research objective was to determine the effect of manure on tuber yield and genetic diversity of the five genotypes of cassava Taluk Kuantan. The research was arranged in the randomized block design (RBD) with two factors. The first factor was five cassava genotypes namely: (IY1) cassava pucuk hitam, (IY 2) cassavabangka, (IY3) cassava pulut, (IY4) cassava roti; and (IY5) cassava pucuk hijau. The second factor was the fertilizer of cow manure consists of five doses, namely: P0 (0 kg/plant), P1 (2 kg/plant), P2 (4 kg/plant), P3 (6 kg/plant); and P4 (8 kg/plant). The parameters observed were morphologicaland agronomiccharacters of cassava, such as plant height,number of tubers/plant, tuber diameter, tuber length, and tuber weight/plant. Pulut cassava produced heaviest tuber in the fertilizer with a dose of 4 kg, bangka and pucuk hijau cassavas produced the highest tuber weight in the fertilizer with 6 kg dose.
OPTIMASI SUHU ANNEALING UNTUK PRIMER g-SSR DAN EST-SSR PADA KACANG HIJAU (Vigna radiata L.) Herman Herman; Lambok Nia Natalya; Suha Maudina Berampu; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 33 No. 1 (2017): Jurnal Dinamika Pertanian Edisi April 2017
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/dp.2017.vol33(1).3821

Abstract

Mungbean (Vigna radiata L.) is one of important legume in Indonesia. G-SSR and EST-SSR markers had been widely used in mungbean genetic diversity research. DNA isolation and DNA amplification are required to obtain genetic information about mungbean to obtain accurate data on the genetic diversity of mungbean. This study aims to determine the Temperature of annealing (Ta) of the g-SSR and EST-SSR primer pairs. The total DNA was isolated from young leaves mungbean origin Pelalawan and the eight primer pairs of the g-SSR and EST-SSR were optimized. The optimal Ta for G2436, G3598, G2516, G7472, G0483. G1671, G3302, and G3427 were 50,55ºC, 51,15ºC,51,25ºC, 51,2ºC, 51,6ºC, 49,0ºC, 49,8ºC, and 52,8ºC respectively. Meanwhile, the optimal Ta for E51985, E19823, E24080, E22860, E26637, E16266, E11659, and E10675 were 52,2ºC, 54,4ºC, 52,5ºC,51,25ºC, 53,25ºC, 54ºC, 54,35ºC, and 53,6ºC respectively.
OPTIMASI SUHU ANNEALING UNTUK EMPAT PRIMER RAPD PADA KACANG HIJAU (Vigna radiata L.) Herman Herman; Martupa Nainggolan; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 34 No. 1 (2018): Jurnal Dinamika Pertanian Edisi April 2018
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/dp.2018.vol34(1).4081

Abstract

Determination of annealing temperature of the primer is the first step for genetic diversity analysis using molecular markers such as RAPD (Random Amplified Polymorphic DNA). This study aims to determine annealing temperature (Ta) of RAPD primers on Kampar Mungbean. Methods included total DNA extraction, electrophoresis, and annealing temperature optimization of four RAPD markers namely OPD-20, OPI-06, OPI-13, dan OPX-13. Optimization was conducted by reducing the Tm value (Time melting) of each primer with 3 (Tm-3) and 5 (Tm-5). The results showed that the optimization using OPD-20 and OPX-13 produced bands at Tm-3 and Tm-5. Meanwhile, optimization using OPI-06 and OPI-13 resulted in bands at Tm-3. The next step was to choose the exact Ta based on the clear and bright band. In conclusion, exact Ta for OPD-20, OPI-06, OPI-13, and OPX-13 were 36,1°C, 38,1°C, 35,4°C, and 32,5°C respectively.
ANALISIS KETAHANAN 10 GENOTIPE KACANG HIJAU (Vigna radiata L.) ASAL PROVINSI RIAU TERHADAP CEKAMAN SALINITAS Imra Atul Uswah; Herman Herman; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 34 No. 1 (2018): Jurnal Dinamika Pertanian Edisi April 2018
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/dp.2018.vol34(1).4083

Abstract

Mung bean (Vigna radiata L.) is one of the important because it has a high nutritional value, so many Indonesia consumption its crop. The production of mung beans is still relatively low due to the reduced availability of land, so it is necessary to use marginal land such as saline land. Therefore, this study aims to analyze the resistance of 10 mung bean genotypes (Vignaradiata L.) from Riau Province on salinity stress. Mung beans seeds of 10 genotypes were grown in polybags with treatments including Control and Treatment saline 7 g/L NaCl of Dolphin salt with 7 replications for 66 HST. The interval of watering saline treatment was five days on time the plant begins to bloom. All genotypes showed different morphological responses to salinity stress. Characteristic of 10 genotypes of mung beans from Riau Province based on salinity stress treatment was obtained a significantly different result on the character of productive branch number, plant height, flowering period, number of pods, pod length, seed number, 100 seed weight, and root length. The tolerant genotype of the salinity of Rohul origin 1 (RU1) was superior to plant height, flowering period, number of pods, number of seeds, and root volume. Genotypes from Pelalawan (P) for branch character, Inhu (IU) genotype was for long pods and 100 seeds, and Rohul 2 (RU2) genotype was for long root characters.
OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI GEN AKTIN PADA PANDAN (Pandanus sp) Larissa Anggisti; Dewi Indriyani Roslim; Herman
DINAMIKA PERTANIAN Vol. 34 No. 2 (2018): Jurnal Dinamika Pertanian Edisi Agustus 2018
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/dp.2018.vol34(2).5411

Abstract

ABSTRACT Genes expression information involved in Pandanus sp. adaptation in Kajuik Lake sp. to environmental stress is unknown. The genes expression analysis requires the internal control gene such as actin. Actin gene is one of the genes that can be expressed continuously at all stages of plant development. Before obtaining genetic information, it is necessary to isolate and amplify DNA. The purpose of this study was to determine the annealing temperature for actin gene amplification in Pandan (Pandanus sp.). The DNA amplification uses two primer pairs with 20 annealing temperatures. The annealing temperatures were calculated based on the mean values of Tm which were then reduced and added with 5, 4, 3, 2 and 1. The annealing temperature of 53.4 ºC (Tm +1) yielded single thick clear using. The annealing temperature of 53.4 ºC (Tm +1) using P_act_F / P_act_R1 primer pair yielded single, thick and firm DNA band. It was concluded that the annealing temperature for actinin gene amplification in Pandanussp was 53.4 ºC with primer P_act_F / P_act_R1. Keywords: Actin gene, Annealing temperatures, PCR
Co-Authors ', Herman ', Tasiah , Deviona, , , HERMAN Abd. Rasyid Syamsuri ALEX HARTANA Andariyusti, Felly Andriani, Lestari Ashfira, Ashfira Asih, Hastini Aslim Rasyad Aslim Rasyad At-Thahirah At-Thahirah Awitdrus Awitdrus, Awitdrus Azrial, Azrial Ciska Vivian Sianturi Defrianto Defrianto Dini Septya Nastiti Dita Deanesia Dodi Frianto, Dodi Endah Budi Lestari Ennie Chahyadi Ennie Chahyadi Faizah, Niswah Fitriani, Ana Fitriyatun Nisa, Fitriyatun Frederika Sinuraya HAJRIAL ASWIDINNOOR Herman Herman - Herman Herman Hestia Hairima Hutagalung, Desriani Ritawati Imra Atul Uswah Ingga Yurisna Fitriani Intan Sari Nuraini Lambok Nia Natalya Larissa Anggisti Martupa Nainggolan Miftahudin . Mutiarawati, Dilla Nery Sofiyanti Nur Aisyah Nurin Nuryani NURKHAIRANI, PUTRI PATMASARI, MUDRIKA Putri Agustina PUTRI PANJAITAN, KHAIRIZA UMAMI Rahmi Anandia Rio Riduan Rosmeilinda, Tio Fanny Roza Elvyra Saktioto Saktioto SARAGIH, JESSICA RODEARNI Siti Khumairoh, Siti Siti Nurhayati Sri Wahyuningsih Suha Maudina Berampu Suhardi Suhardi Suharyanto Suharyanto Utut Suharsono Vera Magdaleni Manullang Wahyu Lestari Wahyu Lestari Yundari, Yundari Yusfi, Liza Aulia